During June, 13-25, the 14th annual Living Cell Course will take place at the University of British Columbia Medicine School (UBC) in Vancouver, Canada. This residential course concentrates on all aspects of the 3D microscopy of living cells. Designed for biological research scientists and advanced graduate students, who apply – or plan to – modern 3D imaging, the course want to open up-to-date methods to a wider selection of scientists. The aim of this intense course is to bring students and manufacturers together. The course’s topics include amongst others scanning systems like AODs, mirrors and disks, deconvolution of wide-field and confocal data, dye design, poisson noise QE and S/N, calcium imaging, as well as „how to keep cells alive“.