Methods and Applications of Fluorescence

August 14, 2009

The 11th International Conference on Methods and Applications of Fluorescence: Spectroscopy, Imaging and Probes will be held in Budapest, Hungary, from September 6-9, 2009. The venue of the Conference is the Congress Center of the oldest Hungarian University, the Eötvös Loránd University.
The meeting will cover the following scientific topics:

– Fluorescence Spectroscopy (Theory and Applications)
– Fluorescence Correlation and Single Molecule Spectroscopy
– Fluorescence in Biology/Medicine: Bioassays, Biophysics
– Special Fluorescent Imaging Techniques: Multi-Photon, Live Cell and Single Molecule Imaging
– Novel Fluorescent Probes, Sensors, Fluorescent Proteins, Quantum Dots, Nanomaterials and their Applications
– Special Fluorescence Techniques: Upconversion, Delayed Fluorescence, Fast Fluorescence Kinetics FRET, etc.
– Fluorescence Microscopy: Towards Higher Spatial and Temporal Resolution
– Fluorescence in Systems Biology High Throughput Screening Assays, Arrays, Micro-chip

Budapest, Hungary

Budapest, Hungary

Principles of Fluorescence Techniques

Juni 24, 2009

The Fluorescence Education Center, also referred to as the Fluorescence Foundation, will host two courses on the principles of fluorescence techniques to be held from:
June 29 – July 2, 2009 in Genova, Italy
September 14-17, 2009 in Madrid, Spain

The Principles of Fluorescence Techniques course will outline the basic concepts of fluorescence techniques and the successful utilization of the currently available commercial instrumentation. The course is designed for students who utilize fluorescence techniques and instrumentation and for researchers and industrial scientists who wish to deepen their knowledge of fluorescence applications. Key scientists in the field will deliver theoretical lectures. The lectures will be complemented by the direct utilization of steady state and lifetime fluorescence instrumentation and confocal microscopy for FLIM and FRET applications.

Topics addressed in this course include:

– Basic Definitions and Principles of Fluorescence
– Fluorescence Polarization
– Time-resolved Fluorescence
– Instrumentation
– Data Manipulation and Data Analysis
– Non-Linear Microscopy Including SHG
– GFP Fluorescence and Photoactivation
– Confocal and Multiphoton Fluorescence Microscopy
– FCS, Fluorescence Correlation Spectroscopy
– FLIM, Fluorescence Lifetime Imaging
– Single Molecule Imaging
– Image Processing and Deconvolution Approaches

Workshop on Single Molecule Spectroscopy

März 26, 2009

For the 15th time now, PicoQuant is hosting the International Workshop on Single Molecule Spectroscopy and Ultrasensitive Analysis in the Life Sciences. The workshop will take place from Sep. 15-18, 2009 in Berlin-Adlershof, WISTA campus, Germany. Topics that will be covered during the talks and a poster session are: Fluorescence Lifetime Correlation Spectroscopy (FLCS), Pulsed Interleaved Excitation (PIE) and Stimulated Emission Depletion Spectroscopy (STED), two-photon excitation, new and robust fluorophores such as quantum dots, metalfluorophore interactions, analysis of living cells, investigation of protein folding and biological function studies of macromolecules and Foerster Resonance Energy Transfer (FRET).

Improving Drug Design

März 6, 2009

The Commonwealth Scientific and Industrial Research Organisation (CSIRO) has patented an improved microscopy method for measuring proteins to help scientists creating new pharmaceuticals for targeted proteins. The method, called Differential Aberration Correction (DAC) microscopy, measures distances at the molecular level in two and three dimensions using conventional fluorescence microscopy.

The leader of CSIRO’s Biotech Imaging team, Dr. Pascal Vallotton, says DAC microscopy measures distances a million times smaller than a tape measure can – in nanometers rather than millimeters. “We want to use our technique to measure accurate dimensions of proteins called membrane receptors. These proteins sit on cell boundaries, acting as gate-keepers, and they represent a class of biomolecules targeted by over 50% of pharmaceuticals”, he says. DAC microscopy is an improvement on an older technology, called FRET. Compared to FRET, DAC measures 1-250 nanometers, giving a more complete picture of drug-membrane receptor interactions. It will complement other techniques like X-ray crystallography. The DAC software was recently demonstrated in the US and will be presented at the Society for Biomolecular Screening conference in Lilles, France in April.

Fluorescence microscopy image of 100nm microspheres used to develop the DAC microscopy method.

Fluorescence microscopy image of 100nm microspheres used to develop the DAC microscopy method.

Focus on Microscopy 2009

Januar 9, 2009

From Sunday April 5 to Wednesday April 8, 2009 the Focus on Microscopy (FOM) conference will take place in Krakow, Poland. It is the continuation of a yearly conference series presenting the latest innovations in optical microscopy and its application in biology, medicine and the material sciences. Key subjects are the theory and practice of 3D optical imaging, related 3D image processing, and reporting especially on developments in resolution and imaging modalities. The FOM conference also covers the rapidly advancing fluorescence labeling techniques for the confocal and multiphoton 3D imaging of live- biological-specimens. A technical exhibition will be a special feature of this year’s conference in Krakow.

Upcoming topics will cover:
– Confocal and multiphoton-excitation microscopy
Novel illumination and detection strategies
– Fluorescence: new labels, fluorescent proteins, quantum dots, single molecule

– Time-resolved fluorescence: FRET, FRAP, FLIM, FCS

– Coherent non-linear microscopy: SHG, THG, SFG, CARS

– Raman, light scattering microscopy

– Multi-dimensional imaging

– Sub-wavelength resolution: near field microscopy, STED, PALM

– Laser manipulation, ablation and microdissection, photoactivation

– Optical tools in genomics, proteomics, phenomics, cytometry

– Magnetic resonance and X-ray microscopy

– Image processing and visualization

– Live cell and whole tissue imaging

The conference will take place at the Jagiellonian University Auditorium Maximum, ul. Krupnicza 35, in the center of Krakow.

Details for registration, abstract submission, deadlines, etc. will soon be available on:

Krakow, Poland, source:

Krakow, Poland (source: