Localization Microscopy Using GFP

Juli 30, 2009

Scientists at Heidelberg University, Germany have developed a new technique for localization microscopy, the “spectral precision distance microscopy” (SPDM). Using visible light, this method allows a single molecule resolution of celullar structures down to the range of few nanometer, about 20 times better than the conventional optical resolution. The researchers invented a new instrument which is a combination of the world’s fastest nano light microscope for 3D cell analysis and the new SPDM technique. Prof. Christoph Cremer of the Kirchhoff Institute of Physics and his team were able to show that SPDM can be realized by common fluorescent dyes, such as the green fluorescent protein (GFP) which can be switched on and off by means of light, as long as certain photophysical conditions are fulfilled. This can be achieved via the so-called “reversible photobleaching” of the dye. So far, only special fluorescent dyes could be used as temporally convertible light signals. According to Cremer there are millions of specimens containing gene constructs with dyes from the GFP group available in biomedical laboratories all over the world. They could be put into immediate use for this new kind of localization microscopy.

Principles of Fluorescence Techniques

Juni 24, 2009

The Fluorescence Education Center, also referred to as the Fluorescence Foundation, will host two courses on the principles of fluorescence techniques to be held from:
June 29 – July 2, 2009 in Genova, Italy
September 14-17, 2009 in Madrid, Spain

The Principles of Fluorescence Techniques course will outline the basic concepts of fluorescence techniques and the successful utilization of the currently available commercial instrumentation. The course is designed for students who utilize fluorescence techniques and instrumentation and for researchers and industrial scientists who wish to deepen their knowledge of fluorescence applications. Key scientists in the field will deliver theoretical lectures. The lectures will be complemented by the direct utilization of steady state and lifetime fluorescence instrumentation and confocal microscopy for FLIM and FRET applications.

Topics addressed in this course include:

– Basic Definitions and Principles of Fluorescence
– Fluorescence Polarization
– Time-resolved Fluorescence
– Instrumentation
– Data Manipulation and Data Analysis
– Non-Linear Microscopy Including SHG
– GFP Fluorescence and Photoactivation
– Confocal and Multiphoton Fluorescence Microscopy
– FCS, Fluorescence Correlation Spectroscopy
– FLIM, Fluorescence Lifetime Imaging
– Single Molecule Imaging
– Image Processing and Deconvolution Approaches


4th CeBiTec Symposium: BioImaging

Juni 18, 2009

Microscopy has contributed immensely to the development of modern biology since 1665 when Robert Hooke published his book „Micrographia“ depicting a large number of microscopical sketches. In our days a major breakthrough in biology is the discovery of the green fluorescent protein (GFP). Another important innovation was found by the german scientist Stefan Hell from Göttingen. He received for example the „Leibniz Preis“ of the German research community (DFG) for „light microscopy with unknown clarity“. These methods enable the visualization of nanoscopic structures in living cells.

Similar high magnification microscopic plus latest electronmicroscopic techniques are also being developed in the department of physics at the University of Bielefeld. A third building block will lead from August 25-28, 2009 from microscopy to imaging.

The topics include:

– Beyond Optical Microscopy
– High Resolution Microscopy in Biology
– From Life Cell Imaging to Systems Biology
– Bioimaging Informatics

The registration is open until July 11, 2009.


bioImaging 2009

bioImaging 2009

Live Cell Imaging at Double the Resolution

Mai 6, 2009

A team of researchers of the University of Georgia (UGA) and the University of California, San Francisco, US has developed a microscope that is capable of live imaging at double the resolution of fluorescence microscopy by using structured illumination. The research was published in Nature Methods on April 26, 2009. “What we’ve done is develop a much faster system that allows you to look at live cells expressing the green fluorescent protein (GFP), which is a very powerful tool for labeling inside the cell,” explained UGA engineer Peter Kner.