Localization Microscopy Using GFP

Juli 30, 2009

Scientists at Heidelberg University, Germany have developed a new technique for localization microscopy, the “spectral precision distance microscopy” (SPDM). Using visible light, this method allows a single molecule resolution of celullar structures down to the range of few nanometer, about 20 times better than the conventional optical resolution. The researchers invented a new instrument which is a combination of the world’s fastest nano light microscope for 3D cell analysis and the new SPDM technique. Prof. Christoph Cremer of the Kirchhoff Institute of Physics and his team were able to show that SPDM can be realized by common fluorescent dyes, such as the green fluorescent protein (GFP) which can be switched on and off by means of light, as long as certain photophysical conditions are fulfilled. This can be achieved via the so-called “reversible photobleaching” of the dye. So far, only special fluorescent dyes could be used as temporally convertible light signals. According to Cremer there are millions of specimens containing gene constructs with dyes from the GFP group available in biomedical laboratories all over the world. They could be put into immediate use for this new kind of localization microscopy.


Light Microscopy Meets Structural Biology

April 21, 2009

A symposium with a focus on light microscopy and its application in structural biology, organized by the European Molecular Biology Laboratory (EMBL) in Heidelberg, Germany will take place form June 22-23, 2009. The symposium aims to bring together structural biologists, cell biologists and light microscopy specialists to explore opportunities and requirements for structural biologists in using different light microscopy techniques and to foster interactions at the interface between structural biology and cell biology.

Planned sessions include:
– Imaging protein-protein interactions
– Protein dynamics
– Correlative light- electron microscopy
– Super-resolution techniques

Deadline for registration is May 3, 2009.

Heidelberg, Germany (source: pixelio.de)

Heidelberg, Germany (source: pixelio.de)

Strengthening the Research and Education Network

März 13, 2009

Microscope designer Leica Microsystems and the European Molecular Biology Laboratory (EMBL) have signed a corporate partnership agreement whereby Leica Microsystems becomes a founder partner of the EMBL Advanced Training Centre scheduled to be completed in September 2009. Besides its financial involvement, the company will provide EMBL with Leica systems and will support events for the international scientific community. In return, Leica will be granted access to the first-class facilities of the molecular biology research institute to run product and application training courses.